human acp5 elisa kit  (Boster Bio)

Journal: Molecular Medicine

Article Title: Inhibition of tartrate-resistant acid phosphatase 5 can prevent cardiac fibrosis after myocardial infarction

doi: 10.1186/s10020-024-00856-1

Figure Lengend Snippet: Inhibition of ACP5 suppresses CF proliferation, migration, and transition into myofibroblasts. ( A ) Relative mRNA expression levels after ACP5 silencing ( n = 3/group). ( B - C ) Western blot analysis of the silencing efficiency of ACP5 ( n = 3/group). ( D - E ) EdU assay was used to detect the cell proliferation rate of CFs ( n = 4/group); scale bar = 100 μm. ( F ) OD values of CFs in different groups ( n = 4/group). ( G - H ) Cell migration was assessed by a wound-healing assay ( n = 4/group); scale bar = 100 μm. ( I - J ) Cell migration was assessed by Transwell assays ( n = 4/group); scale bar = 100 μm. ( K - N ) Relative mRNA expression levels of ACP5, α-SMA, COL1, and COL3 in CFs in different groups ( n ≥ 3/group). ( O - Q ) Western blot analysis of α-SMA and COL1 ( n = 3/group). * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: P > 0.05

Article Snippet: The Mouse ACP5 ELISA Kit was purchased from Signalway Antibody (USA, #EK12408), and the Human ACP5 ELISA Kit was purchased from BOSTER (China, #EK2138).

Techniques: Inhibition, Migration, Expressing, Western Blot, EdU Assay, Wound Healing Assay

Journal: Molecular Medicine

Article Title: Inhibition of tartrate-resistant acid phosphatase 5 can prevent cardiac fibrosis after myocardial infarction

doi: 10.1186/s10020-024-00856-1

Figure Lengend Snippet: Overexpression of ACP5 promotes CF proliferation, migration, and transition into myofibroblasts. ( A ) Relative mRNA expression levels of ACP5 and the overexpression efficiency ( n = 4/group). ( B - C ) Western blot analysis of the overexpression efficiency of ACP5 ( n = 3/group). ( D - E ) The EdU assay was used to detect the cell proliferation rate of CFs ( n = 4/group); scale bar = 100 μm. ( F ) OD values of CFs in different groups ( n = 3/group). ( G - H ) Cell migration was assessed by a wound-healing assay ( n = 4/group); scale bar = 100 μm. ( I - J ) Cell migration was assessed by Transwell assays ( n = 4/group); scale bar = 100 μm. ( K - N ) Relative mRNA expression levels of ACP5, α-SMA, COL1, and COL3 in CFs in different groups ( n = 4/group). ( O - Q ) Western blot analysis of α-SMA and COL1 ( n = 3/group). * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: P > 0.05

Article Snippet: The Mouse ACP5 ELISA Kit was purchased from Signalway Antibody (USA, #EK12408), and the Human ACP5 ELISA Kit was purchased from BOSTER (China, #EK2138).

Techniques: Over Expression, Migration, Expressing, Western Blot, EdU Assay, Wound Healing Assay

Journal: Molecular Medicine

Article Title: Inhibition of tartrate-resistant acid phosphatase 5 can prevent cardiac fibrosis after myocardial infarction

doi: 10.1186/s10020-024-00856-1

Figure Lengend Snippet: ACP5 inhibition suppresses fibrosis in MI mice and enhances cardiac function. ( A ) Masson staining and Sirius red staining in each group ( n = 6/group; upper layer, scale bar = 1000 μm; middle layer, scale bar = 100 μm; lower layer, scale bar = 100 μm). ( B ) Masson staining of fibrosis. ( C ) Sirius red staining of the collagen area. ( D ) Representative images of echocardiography. ( E - F ) Echocardiographic measurements of LVEF (E) and LVFS (F) ( n = 6/group). ( G - I ) Western blot analysis of α-SMA and COL1 in the hearts of mice in different groups ( n = 3/group). ( J - K ) Immunofluorescence staining of α-SMA in the hearts of mice in different groups ( n = 4/group); scale bar = 50 μm. (The bottom-layer image in Fig. 4J is the locally enlarged image circled in the Merge graph, bar = 20 μm). * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: P > 0.05

Article Snippet: The Mouse ACP5 ELISA Kit was purchased from Signalway Antibody (USA, #EK12408), and the Human ACP5 ELISA Kit was purchased from BOSTER (China, #EK2138).

Techniques: Inhibition, Staining, Western Blot, Immunofluorescence

Journal: Molecular Medicine

Article Title: Inhibition of tartrate-resistant acid phosphatase 5 can prevent cardiac fibrosis after myocardial infarction

doi: 10.1186/s10020-024-00856-1

Figure Lengend Snippet: ACP5 affects the GSK3β/β-catenin signal transduction pathway. ( A - D ) Western blot analysis of the expression levels of ACP5, p-GSK3β, GSK3β, and β-catenin in ACP5-deficient CFs ( n = 3/group). ( E - H ) Western blot analysis of the expression levels of ACP5, p-GSK3β, GSK3β, and β-catenin in CFs overexpressing ACP5 ( n = 3/group). ( I - L ) Western blot analysis of the expression levels of ACP5, p-GSK3β, GSK3β, and β-catenin in the hearts of mice in different groups ( n ≥ 3/group). * P < 0.05, ** P < 0.01, *** P < 0.001, and ns: P > 0.05

Article Snippet: The Mouse ACP5 ELISA Kit was purchased from Signalway Antibody (USA, #EK12408), and the Human ACP5 ELISA Kit was purchased from BOSTER (China, #EK2138).

Techniques: Transduction, Western Blot, Expressing

Journal: Molecular Medicine

Article Title: Inhibition of tartrate-resistant acid phosphatase 5 can prevent cardiac fibrosis after myocardial infarction

doi: 10.1186/s10020-024-00856-1

Figure Lengend Snippet: ACP5 affects CF activation by regulating ERK. ( A - B ) Western blot analysis of the expression levels of p-ERK and ERK in ACP5-deficient CFs ( n = 3/group). ( C - D ) Western blot analysis of the expression levels of p-ERK and ERK in CFs overexpressing ACP5 ( n = 3/group). ( E - F ) Western blot analysis of the expression levels of p-ERK and ERK in the hearts of mice in different groups ( n = 3/group). ( G - K ) Western blot analysis of the expression levels of ACP5, p-ERK, ERK, p-GSK3β, GSK3β, and β-catenin in CFs pretreated with Ro 67-7476 (an ERK agonist) ( n = 3/group). ( L - M ) The EdU assay was used to detect the proliferation rate of CFs pretreated with Ro 67-7476 (an ERK agonist) ( n = 4/group); scale bar = 100 μm. ( N - O ) Cell migration was assessed by Transwell assays ( n = 4/group); scale bar = 100 μm. ( P - R ) Western blot analysis of α-SMA and COL1 in the hearts of mice in different groups ( n = 3/group).* P < 0.05, ** P < 0.01, *** P < 0.001, and ns: P > 0.05

Article Snippet: The Mouse ACP5 ELISA Kit was purchased from Signalway Antibody (USA, #EK12408), and the Human ACP5 ELISA Kit was purchased from BOSTER (China, #EK2138).

Techniques: Activation Assay, Western Blot, Expressing, EdU Assay, Migration

Journal: Molecular Medicine

Article Title: Inhibition of tartrate-resistant acid phosphatase 5 can prevent cardiac fibrosis after myocardial infarction

doi: 10.1186/s10020-024-00856-1

Figure Lengend Snippet: Schematic diagram of the mechanism of ACP5 in myocardial fibrosis after MI. Under the stimulation of MI or Ang II, the increased expression of ACP5 activates the ERK/GSK3β/β-catenin signaling pathway, which promotes the transformation of CFs into myofibroblasts with more active proliferation, migration and fibrosis, leading to the onset of myocardial fibrosis. (Generated by Figdraw)

Article Snippet: The Mouse ACP5 ELISA Kit was purchased from Signalway Antibody (USA, #EK12408), and the Human ACP5 ELISA Kit was purchased from BOSTER (China, #EK2138).

Techniques: Expressing, Transformation Assay, Migration, Generated